J. Mex. Chem. Soc. 2006, 50(3), 96-99
© 2006, Sociedad Química de México
ISSN 1870-249X
Article
Rospiglioside, a New Totarane Diterpene from the Leaves
of Retrophyllum rospigliosii
Juan M. Amaro-Luis,* Ángel E. Amesty, Roger Montealegre and Alí Bahsas
Laboratorio de Productos Naturales, Departamento de Química, Facultad de Ciencias, Universidad de Los Andes (ULA), Mérida,
Estado Mérida, Venezuela-5101, Tel.: 58-274-2401385; FAX: 58-274-2443909; E-mail: jamaro@ula.ve
Dedicated to Professor Pedro Joseph-Nathan on the occasion of his 65Th birthday
Recibido el 23 de enero del 2006; aceptado el 20 de marzo del 2006.
Abstract. A new glucosyl totarane diterpene named rospiglioside,
together with seven known abietane and totarane diterpenes identified
as ferruginol, sugiol, sugiol acetate, totarol, totarol acetate, 4β-carboxy-19-nortotarol and 16-hydroxy-4β-carboxy-19-nortotarol have
been isolated from the leaves of Retrophyllum rospigliosii (Pilger) C.
N. Page (Podocarpaceae). The structure of rospiglioside was established on the basis of spectroscopic methods, mainly 1D- and 2DNMR experiments.
Keywords: Retrophyllum rospigliosii, Podocarpaceae, diterpenes,
abietane, totarane
Resumen. De las hojas del Retrophyllum rospigliosii (Pilger) C. N.
Page (Podocarpaceae) fue aislado un nuevo glucosil diterpeno de la
serie del totarano denominado rospigliósido, y siete diterpenos conocidos de las series del abietano y del totarano identificados como
ferruginol, sugiol, acetato de sugiol, totarol, acetato de totarol, 4βcarboxi-19-nortotarol y 16-hidroxi-4β-carboxi-19-nortotarol. La
estructura del rospigliósido fue establecida sobre la base de métodos
espectroscópicos, principalmente experimentos de RMN uni- y bidimensionales
Palabras clave: Retrophyllum rospigliosii, Podocarpaceae, diterpenos, abietano, totarano
Introduction
by the Artemia salina test [4], to give eight pure compounds.
The seven known diterpenes, whose identity was established
by 1D- and 2D-NMR studies and comparison of their spectral
data with those reported in the literature, were shown to be
totarol (2) [5], totarol acetate (3) [6], 4b-carboxy-19-nortotarol
(4) [5,7], 16-hydroxy-4b-carboxy-19-nortotarol (5) [8], ferruginol (6) [9], sugiol (7) [10, 11] and sugiol acetate (8) [12].
Rospiglioside (1) was obtained as an oil, [a] D: -34.0º
(MeOH), and its HR-EIMS established the molecular formula
C 26H 38O 8 (m/z: 478.2523 [M +]). Its IR spectrum showed
absorption bands of hydroxyl groups (3418 cm-1), a carbonyl
group (1722 cm-1) and an aromatic ring (1636, 814 cm-1). The
1
H NMR of 1 indicated the presence of two aromatic orthocoupled protons [doublets at δ 6.62 and δ 6,94 (J = 8.6 Hz )],
two tertiary methyl groups (singlets at δ 1.09 y δ 1.32) and an
isopropyl group, attached to an aromatic ring (doublet at δ
1,33 (6H) and septet at δ 3.30 (1H, J = 7.2 Hz)); this last
group was also characterized in the 1H,1H-COSY spectrum,
which showed a cross-peak between the H-15 benzylic
methine septet and the doublet of both H 3-16 and H 3-17
methyl groups.
A sharp doublet of an anomeric proton at δ 5.54 (J = 8.1
Hz) and several overlapped multiplets in the range 3.30-3.74
ppm were also observed in the 1H NMR spectrum, indicating
the presence of a glucopyranosyl moiety in the molecule. The
large value for the splitting (J = 8.1 Hz) of the glucosyl
anomeric proton doublet, due to a diaxial coupling between H1’ and H-2’, suggested a β-configuration [13]. This was also
confirmed in the 13C NMR spectrum by the position of C-1’ (δ
94.1), which agreed with that expected for a β-D-gluco-pyranosyl residue [14].
Retrophyllum rospigliosii (Pilger) C. N. Page, also known as
Decussocarpus rospigliosii (Pilger) De Laubenfels is a large
tree growing in the Andean Region of Colombia, Ecuador and
Venezuela. This tree, popularly known in Venezuela as “Pino
Laso”, has a notable ecological interest, since it is one of the
few native conifers of the Andean rain forest. Its wood, valued
for its durable heartwood, has potential in cabinetmaking and
decorative works [1].
In previous communications we reported the isolation and
characterization of several phenolic diterpenes of abietane and
totarane groups and also some norditerpene dilactones from
bark of Retrophyllum rospigliosii (at that time named
Decussocarpus rospigliosii) [2, 3]. In a continuation of our
studies, we now report the isolation and structure elucidation
of a new glucosyl totarane diterpene, rospiglioside (1), together with seven known diterpenes, four of which (2-5) possess a
totarane skeleton, and the other three ones (6-8) an abietane
skeleton.
Results and discussion
The acetone extract of leaves of R. rospigliosii was preadsorbed on silica gel and sequentially extracted with hexane,
CH2Cl2, EtOAc and MeOH in a soxhlet. The concentrate of
CH2Cl2 extract was fractioned on a vacuum liquid chromatography column (VLCC) and the obtained fractions were rechromatographed on silica gel and Sephadex LH-20 (see experimental). The separation of the processed fractions was guided
Rospiglioside, a New Totarane Diterpene from the Leaves of Retrophylumm rospigliosii
Apart from six signals typical of hexose, the 13C NMR
spectrum of 1 shows twenty additional carbon signals; the
DEPT spectra revealed that there were four methyls, five
methylenes, four methines including two aromatic sp2 carbons,
and seven quaternary carbons including a carboxyl and four
aromatic carbons. On the basis of the analysis of 1H,1H-COSY,
HMQC and HMBC spectra, all proton and carbon signals of 1
were assigned (Table 1).
The HMBC spectrum provided information about the
location of functional groups in the molecule, establishing a
totarane structure for 1. In effect, C-16 and C-17 methyl protons (δ 1.33) correlate with a methine carbon (δ 27.1; C-15)
and a quaternary aromatic carbon (δ 130.1; C-14), which at the
same time shows connection with an aromatic (δ 6.62; H-12)
and two benzyls (δ 2.93 and δ 2.61; H-7) protons; the H-12
aromatic proton also correlates with an oxygenated aromatic
carbon (δ 153.3; C-13) and this one has a 3J long-range coupling with a second aromatic hydrogen (δ 6.94; H-11), thus
confirming the partial structure of ring C for a totarane skeleton. This spectrum also shows the location, in the A/B decalin
97
system, of an angular methyl group whose protons (δ 1.09; H20) have long-range correlation with a methylene carbon (δ
40.2; C-1), an aromatic quaternary carbon (δ 139.8; C-9) and a
methine carbon (δ 52.3; C-5). The hydrogen of this methine (δ
1.46; H-5) correlates with a tetrasubstituted sp3 carbon (δ 43.7;
C-4), a methyl carbon (δ 27.7 C-18), a carboxyl carbon (δ
175.6; C-19) and three methylene carbons, [δ 37.3 (C-3); δ
21.2 (C-6) and δ 29.5 (C-7)], thus establishing a spin system
composed of a methine (C-5) and two adjacent methylene
groups (C-6/C-7), located between a quaternary aromatic carbon (C-8) and a fully substituted sp3 carbon (C-4) which bears
both, a methyl (C-18) and a carboxyl group (C-19). The
observation in the HMBC spectrum of a 3J long-range correlation between C-19 (δ 175.6) and the anomeric proton (δ 5.54;
H-1’) clearly established that the glucosylation site was on C19-carboxyl group. Finally the configuration of this glucosylcarboxy group must be β-axial on the basis of the C-18
methyl and C-19 carbonyl carbons chemical shift values,
which are in agreement with literature data [15]. From these
results, the structure of compound 1 was concluded to be 4β-
Table 1. 1H NMR (400 MHz) and 13C NMR (100 MHz) spectral data and C-H correlations in HMBC spectrum of 1.
Nº
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
1’
2’
3’
4’
5’
6’
1H
NMR
α 1.27 (m)
β 2.34 (dt, J= 12.5 and 3.5)
α 2.10 (m)
β 1.55 (dtt, J= 12.5, 12.8 and 3.5)
α 1.12 (m)
β 2.23 (dt, J= 12.5 and 3.1)
1.46 (δ, J = 12.0)
α 2.29 (m)
β 2.04 (m)
α 2.61 (m)
β 2.93 (dd, J= 16.6 and 4.5)
6.94 (d, J= 8.6 )
6.62 (d, J = 8.6)
3.30 (spt, J = 7.2)
1.33 (d, J = 7.2)
1.33 (d, J = 7.2)
1.32 (s)
1.09 (s)
5.54 (d, J = 8.1)
3.45 (m)
3.35 (m)
3.40 (m)
3.35 (m)
3.65 and 3.81 (m)
* Spectra recorded in (CD3)2CO, ppm from TMS
13C
HMBC(C → H)
NMR
40.2 (t)
H-20
19.9 (t)
H-1α
37.3 (t)
H-1β, H-5, H-18
43.7 (s)
52.3 (d)
21.2 (t)
H-3α, H-5, H-18
H-3β, H-6α, H-6β, H-7β, H-18, H-20
H-5, H-7α, H-7β
29.5 (t)
H-5, H-6α, H-6β
133.4 (s)
139.8 (s)
38.3 (s)
123.8 (d)
114.2 (d)
153.3 (s)
130.1 (s)
27.1 (d)
19.7 (q)
19.5 (q)
27.7 (q)
175.6 (s)
23.1 (q)
94.1 (d)
72.9 (d)
77.2 (d)
70.3 (d)
77.2 (d)
61.8 (t)
H-6α, H-7α, H-7β, H-11
H-7α, H-7β, H-12, H-20
H-1α, H-6β, H-11, H-20
NC
NC
H-11, H-12
H-7α, H-7β, H-12, H-16, H-17
H-16, H-17
H-17
H-16
H-3α, H-5
H-3α, H-5, H-18, H-1’
H-1α, H-5,
H-2’, H-3’, H-5’
H-3’, H-4’
H-1’, H-2’, H-3’, H-5’
H-2’, H-3’, H-5’, H-6’
H-1’, H-3’, H-4’, H-6’
H-4’, H-5’
98
J. Mex. Chem. Soc. 2006, 50(2)
carboxy-O-β-glucopyranosyl-19-nortotarol. Since this is a
new diterpenes glucoside, it has been assigned the trivial
name rospiglioside.
In preliminary biological tests, rospiglioside showed significant cytotoxic activity against Artemia salina. Currently,
we are continuing studies directed to evaluate its potential
effects on seed germination and root and shoot growth of lettuce (Lactuca sativa).
Experimental
General Experimental Procedures. Melting points were
determined with a Fisher-Johns apparatus and they have not
been corrected. Optical activities were measured in CHCl3 on
a Rudolph Research Autopol III polarimeter. IR spectra were
recorded on a Perkin-Elmer FT-1725X spectrophotometer as
film or KBr pellets. 1H-, 13C- and two-dimensional NMR spectra were acquired with a Bruker-Avance DRX-400 instrument,
using CDCl3 as solvent. EI-MS and HREI-MS were run on a
Hewlett-Packard 5930A and on an Autospec VG spectrometer,
respectively; direct inlet, 70 eV. TLC was carried out on 0.25
mm layers of silica gel PF 254 (Merck). VCC was performated
with silica gel 60 (70-230 mesh.).
Plant material. The leaves of Retrophyllum rospigliosii
(Pilger) C. N. Page were collected in La Carbonera, Municipio
Autónomo Andrés Bello, Estado Mérida, Venezuela, in May
2000. A voucher specimen (J. M. Amaro-Luis, Nº 1630) was
deposited at Herbarium MERF of Pharmacy Faculty,
University of Los Andes.
Extraction and separation. Dried and pulverized leaves of R.
rospigliosii (ca. 3.6 Kg) were extracted at room temperature
with acetone and then with MeOH in a soxhlet to give, respectively, 548 and 144 g of crude extracts. The acetone extract
was preadsorbed on silica gel and sequentially extracted with
Juan M. Amaro-Luis et al.
hexane, CH2Cl2, EtOAc and MeOH in a soxhlet. The CH2Cl2
fraction, after evaporation under reduced pressure, give 42 g of
residue, which was chromatographed (VLC) over silica gel 60,
eluting with hexane and EtOAc in mixtures of increasing
polarity. Fractions of 500 mL were collected and combined for
TLC similarity into eight major fractions (A-H). Fractions B
was rechromatographed on a silica gel column using hexaneEtOAc (9:1) to furnish compounds 2 (320 mg), 3 (33 mg) and
8 (164 mg) (45 mg). Fraction C was applied to repeated silica
gel (hexane-EtOAc 4:1 y 7:3) and Sephadex LH-20 (hexaneCH2Cl2-MeOH 1:1:1) column chromatography to yield the
compounds 4 (120 mg), 6 (42 mg) and 7 (182 mg). Fraction D
was filtered through a Sephadex LH-20 (hexane-CH 2Cl 2MeOH 1:2:1) and several subfractions were further separated
by PTLC on silica gel (eluted with hexane-EtOAc 7:3, developed 2x) giving 4 (83 mg) and 5 (45 mg). Fraction E was subjected to a column of Sephadex LH-20 eluted with HexaneCH2Cl2-MeOH (1:2:2); subfractions 12-15 were combined and
purified by PTLC (silica gel, hexane-EtOAc 1:1) to give compound 1 (35 mg).
Identification of known compounds. Compounds 2-8 were
identified by comparison of their physical constants and their
1
H- and 13C NMR data with those reported in literature for
totarol (2) (m.p. 130-132 ºC; [α]D +43º), totarol acetate (3)
(m.p. 120-122 ºC), 4β-carboxy-19-nortotarol (4) (m.p. 180182 ºC; [α]D + 118º), 16-hydroxy-4β-carboxy-19-nortotarol
(5) (m.p. 108-110 ºC; [α]D + 77º ), ferruginol (6) (m.p. 163165 ºC; [α]D +55º), sugiol (7) (m.p. 294-296 oC; [α]D + 23º)
and sugiol acetate (8) (m.p. 165-166 oC; [α]D + 27º).
Rospiglioside (1). Colorless oil; [α]D -34.0º (MeOH, c 0.45);
HR-EIMS [M + ] m/z 478.2523 (requires for C 26 H 38 O 8 ,
478.2567); UV (MeOH) λmax 283 nm (log ε 3.55); IR (film)
νmax 3418, 2870, 2930, 1722 1600, 1636, 1074, 1028, 814; 1H
and 13C NMR data, see Table 1; LR-EIMS, m/z (rel. int.): 478
[M]+ (54), 316 [M-Gluc.]+ (23), 301 [M-Gluc.-CH3]+ (100), 271
Rospiglioside, a New Totarane Diterpene from the Leaves of Retrophylumm rospigliosii
[M- Gluc.-COOH] + (5), 255 [M-Gluc.-CH 3-COOH] + (26),
213(24), 201 (5), 289 (6), 175 (7), 173 (5), 157 (10), 149 (7),
109(7), 95 (8), 91 (9), 81 (11), 73 (17), 60 (26).
Bioassay results. Artemia salina test was performed according to Meyer et al. methodology [4].
Acknowledgements
The authors are grateful to FONACIT (National Fund of
Science, Technology and Innovation) for financial support
(Grant S1-970001521). We also would like to thank Prof
Angel G. Ravelo, Instituto Universitario de Bio-Orgánica,
Universidad de La Laguna. Tenerife, Spain, for the HR-EIMS.
References
1. Veillon, J. P. Coniferas Autóctonas de Venezuela: Los
Podocarpus. Ed. Universidad de Los Andes, Mérida, Venezuela,
1962, 31-33.
99
2. Amaro-Luis, J. M.; Carroz, D. J. Nat. Prod. 1988, 51, 1249-1250.
3. Amaro-Luis, J. M.; Carroz, D. Rev. Lat. Quím. 1989, 20, 8-10.
4. Meyer, B. N.; Ferrigni, N. R.; Putmann, J. E.; Jacobson, L. B.;
Nichols, D. E.; McLaughlin, J. L. Planta Med. 1982, 45, 31-34.
5. Ying, B.-P.; Kubo, I. Phytochemistry 1991, 30, 1951-1955.
6. Matsumoto, T.; Suetsugu, A. Bull. Chem. Soc. Japan 1979, 52,
1450-1453.
7. De Paiva Campello, J.; Ferreira Fonseca, S.; Chang, C.-J.;
Wenkert, E. Phytochemistry 1975, 14, 243-248.
8. Park, H.-S.; Kai N.; Fukaya H.; Aoyagi Y; Takeya K.
Heterocycles 2004, 63, 347-357.
9. Tezuka, Y.; Kasimu, R.; Li J. X.; Basnet, P.; Tanaka, K.; Namba,
T.; Kadota, S. Chem. Pharm. Bull. 1998, 46, 107-112.
10. Ara, I.; Siddiqui B. S.; Faizi, S.; Siddiqui, S. J. Nat. Prod. 1988,
51, 1054-1061.
11. Chen, W.; Meng Q.; Piantini, U.; Hesse, M. J. Nat. Prod. 1989,
52, 581-87.
12. Joland, S. D.; Hoffmann, J. J.; Schram, K. H.; Cole, J. R.; Bates,
R. B.; Tempesta, M. S. J. Nat. Prod. 1984, 47, 983-87.
13. Bock, K.; Thoegersen, H. Ann. Rep. NMR Spectrosc. 1982, 13, 157.
14. Agrawal, P. K. Phytochemistry 1992, 31, 3307-3330.
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203-209.
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